Here's what some marvel editors had picked.

http://www.marvel.com/news/comicstories.2681.TGIF%7Ecolon%7E_Spider-Man%27s_Villains

Me? I tend to gravitate towards the Green Goblin and the Hobgoblin.

Other spidey villains that i like: The Shocker, Mysterio, Electro, Venom, Carnage, Black Cat, Chameleon and ok, Doctor Octopus too. :P

How about you guys?

Spidey has one of the most colorful and most memorable rogues gallery ever.

Venom, Kingpin, Doc Ock are my favs. Never liked shocker, and there are too many `gliding` villains: Green Goblin, Hobgoblin, Jack O`Lantern and that new guy from BND.

Hahaha, this article was funny.

QuoteDon't they know that nothing can break up Peter and MJ...wait a minute...ummm...anyways...my favorite of all Spidey villains is the McFarlane era Venom.

QuoteI recall that when I was working on DAREDEVIL and Frank Miller was bringing Wilson Fisk into that mythology I begged Frank to have the Kingpin use his obliterator cane, but Frank didn't listen to me. And that's why he never amounted to anything in this business.

QuoteSwarm. Who is he? Fritz von Meyer, a Nazi scientist and world expert in toxic poisons, who looted European 
Swarm

Capitalists to fund the research that eventually turned him into a living swarm of bees. Yes, a poison-expert Nazi whose body was made of bees. You don't get more evil than that. Try it. Attila the Hun made out of cockroaches? Pretty evil, but come on—Nazis, poison...bees?! In a universe full of villains whose points of view you can understand and motives you respect, how great is it to have a guy you couldn't possibly root for under any circumstance?

Swarm, baby. He's where it's at.

I can't decide...I love so many!  Spidey has a great lineup of baddies.

Green Goblin: I just love villains who devote their lives to destroying the hero.  In the end, Osborne didn't care about money, power, prestige.  It all boiled down to making Peter Parker's life a living hell.

Doc Ock: Those tentacles are just so freaking cool!

Venom: Same as Gobby, but he's actually got a sympathetic side.  Gobby knows he's evil and doesn't care.  Venom (well, Brock Venom) seriously thinks he's a decent guy.  And when Spidey isn't there to screw with him, he actually can be.

Mysterio: Um.  YES.  Nobody is cooler than this guy.

Shocker: I just love this dude's costume and shock bracelets.

i think spidey has the best villians!my favorites are rhino,sandman,electro,and the 1st green goblin

Doc Ock and Carnage

I liked Silvermane, and Man Mountain Marko.

Its hard to choose but id go with The Shocker, Venom and Tombstone, i've ALWAYS loved those.

The Shocker: He's always been the dangerous proffesional villain who doesnt live to kill spider-man.

Venom: Well,  Eddie Brock was awesome because he was always the guy spidey couldnt beat.

Tombstone: Lonnie didnt just target Spidey but also Robbie, i kinda like that everyone isnt just hunting peter but his friends also has enemies.

Carnage, easily. But then again, I've always been somewhat biased towards psychotic serial-killing villains.

Electro.